Comparative study of the analgesic effects of Bungarus fasciatus snake venom from Vinh Phuc and Tien Giang Provinces of Vietnam

Purpose: To determine the analgesic activity of Bungarus fasciatus venoms and their fractions from two Vietnamese Provinces.
 Methods: Male Swiss Albino mice were randomly divided into three groups containing 8 to 10 each. Control group was injected subcutaneously with normal saline, standard received aspirin solution (50 mg/kg) perorally, study a crude venom or isolated in physiological saline. activity, acetic acid writhing tail immersion tests used. The separated by liquid chromatography analyzed.
 Results: Both showed effect test, but only Tien Giang test. bioactive Vinh Phuc significantly different, most being more active (p < 0.05). Thus, 35 min after injection, number writhings decreased 15 - 16 control 0.85 ± 0.34 for BF-4VS (Vinh Phuc) fraction compared 2.67 1.20 0.05) BF-4DT (Thien Giang) fraction. Two proteins venom, one greater matched known B. phospholipase A2.
 Conclusion: samples different provinces Vietnam reveal that pharmacological profiles differ. isolates can be explored as leads development new agents.